Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3992488..3993182 Replicon chromosome
Accession NZ_CP116046
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag PIH14_RS19290 Protein ID WP_001263493.1
Coordinates 3992784..3993182 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID -
Locus tag PIH14_RS19285 Protein ID WP_032162515.1
Coordinates 3992488..3992781 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS19265 (3988120) 3988120..3988617 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PIH14_RS19270 (3988841) 3988841..3990553 - 1713 Protein_3754 flagellar biosynthesis protein FlhA -
PIH14_RS19275 (3990525) 3990525..3991310 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
PIH14_RS19280 (3991381) 3991381..3992436 + 1056 WP_032162516.1 DNA polymerase IV -
PIH14_RS19285 (3992488) 3992488..3992781 + 294 WP_032162515.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIH14_RS19290 (3992784) 3992784..3993182 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIH14_RS19295 (3993192) 3993192..3993644 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
PIH14_RS19300 (3993890) 3993890..3994093 + 204 Protein_3760 RtcB family protein -
PIH14_RS19305 (3994092) 3994092..3994613 + 522 Protein_3761 peptide chain release factor H -
PIH14_RS19310 (3994670) 3994670..3996127 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
PIH14_RS19315 (3996388) 3996388..3996846 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3997442) 3997442..3997522 + 81 NuclAT_10 - -
- (3997442) 3997442..3997522 + 81 NuclAT_10 - -
- (3997442) 3997442..3997522 + 81 NuclAT_10 - -
- (3997442) 3997442..3997522 + 81 NuclAT_10 - -
PIH14_RS19320 (3996938) 3996938..3998182 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T267741 WP_001263493.1 NZ_CP116046:3992784-3993182 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11261.86 Da        Isoelectric Point: 5.9050

>AT267741 WP_032162515.1 NZ_CP116046:3992488-3992781 [Escherichia coli]
MHRILAEKSVNITELRKNPVKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure

References