Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4358662..4359461 Replicon chromosome
Accession NZ_CP116044
Organism Escherichia coli strain STEC1575

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag PHA55_RS21225 Protein ID WP_000347273.1
Coordinates 4358997..4359461 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PHA55_RS21220 Protein ID WP_001307405.1
Coordinates 4358662..4358997 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA55_RS21205 (4354447) 4354447..4355217 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
PHA55_RS21210 (4355233) 4355233..4356567 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PHA55_RS21215 (4356942) 4356942..4358513 + 1572 WP_001273741.1 galactarate dehydratase -
PHA55_RS21220 (4358662) 4358662..4358997 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PHA55_RS21225 (4358997) 4358997..4359461 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PHA55_RS21230 (4359516) 4359516..4360325 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
PHA55_RS21235 (4360574) 4360574..4361854 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
PHA55_RS21240 (4361877) 4361877..4362350 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PHA55_RS21245 (4362361) 4362361..4363140 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PHA55_RS21250 (4363130) 4363130..4364008 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
PHA55_RS21255 (4364026) 4364026..4364460 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4347672..4359461 11789


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T267720 WP_000347273.1 NZ_CP116044:4358997-4359461 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT267720 WP_001307405.1 NZ_CP116044:4358662-4358997 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References