Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3903210..3903807 Replicon chromosome
Accession NZ_CP115707
Organism Klebsiella pneumoniae strain CPE16

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag PGD48_RS19165 Protein ID WP_004142563.1
Coordinates 3903490..3903807 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag PGD48_RS19160 Protein ID WP_004142561.1
Coordinates 3903210..3903497 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PGD48_RS19130 (3899290) 3899290..3899538 + 249 WP_002893037.1 DUF1158 domain-containing protein -
PGD48_RS19135 (3899556) 3899556..3899897 - 342 WP_159174530.1 RamA family antibiotic efflux transcriptional regulator -
PGD48_RS19140 (3899928) 3899928..3901043 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
PGD48_RS19145 (3901223) 3901223..3901804 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
PGD48_RS19150 (3901804) 3901804..3902172 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
PGD48_RS19155 (3902292) 3902292..3902942 + 651 Protein_3760 oxygen-insensitive NAD(P)H nitroreductase -
PGD48_RS19160 (3903210) 3903210..3903497 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
PGD48_RS19165 (3903490) 3903490..3903807 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PGD48_RS19170 (3903992) 3903992..3905035 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
PGD48_RS19175 (3905702) 3905702..3906568 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
PGD48_RS19180 (3906677) 3906677..3908104 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T267310 WP_004142563.1 NZ_CP115707:c3903807-3903490 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT267310 WP_004142561.1 NZ_CP115707:c3903497-3903210 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References