Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2472113..2472636 Replicon chromosome
Accession NC_017349
Organism Staphylococcus aureus subsp. aureus LGA251

Toxin (Protein)


Gene name yoeB Uniprot ID X5DYX7
Locus tag SARLGA251_RS12405 Protein ID WP_000074048.1
Coordinates 2472113..2472379 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A2S6D1W5
Locus tag SARLGA251_RS12410 Protein ID WP_000587617.1
Coordinates 2472379..2472636 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SARLGA251_RS12385 2467332..2468519 - 1188 WP_000026189.1 MFS transporter -
SARLGA251_RS12390 2468914..2469690 - 777 WP_000072160.1 iron export ABC transporter permease subunit FetB -
SARLGA251_RS12395 2469683..2470345 - 663 WP_000923508.1 ATP-binding cassette domain-containing protein -
SARLGA251_RS12400 2470690..2471757 - 1068 WP_001076685.1 M42 family metallopeptidase -
SARLGA251_RS12405 2472113..2472379 - 267 WP_000074048.1 Txe/YoeB family addiction module toxin Toxin
SARLGA251_RS12410 2472379..2472636 - 258 WP_000587617.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
SARLGA251_RS12420 2472996..2473451 + 456 WP_000732082.1 DUF1307 domain-containing protein -
SARLGA251_RS12425 2473619..2475196 - 1578 WP_000143506.1 FMN-binding glutamate synthase family protein -
SARLGA251_RS12430 2475390..2476139 + 750 WP_000072456.1 CPBP family intramembrane metalloprotease -
SARLGA251_RS12435 2476363..2477556 - 1194 WP_000675399.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10648.23 Da        Isoelectric Point: 10.1384

>T26724 WP_000074048.1 NC_017349:c2472379-2472113 [Staphylococcus aureus subsp. aureus LGA251]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T26724 NC_017349:c2472379-2472113 [Staphylococcus aureus subsp. aureus LGA251]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9747.71 Da        Isoelectric Point: 4.1353

>AT26724 WP_000587617.1 NC_017349:c2472636-2472379 [Staphylococcus aureus subsp. aureus LGA251]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDSSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT26724 NC_017349:c2472636-2472379 [Staphylococcus aureus subsp. aureus LGA251]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7V6W
PDB 7V5Y
PDB 7V5Z

Antitoxin

Source ID Structure
AlphaFold DB A0A2S6D1W5

References