Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 4503664..4504703 Replicon chromosome
Accession NZ_CP115665
Organism Pseudomonas putida strain PD584

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag PED37_RS20500 Protein ID WP_010954922.1
Coordinates 4504128..4504703 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag PED37_RS20495 Protein ID WP_049588231.1
Coordinates 4503664..4504131 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PED37_RS20480 (PED37_20480) 4499816..4500889 + 1074 WP_010954918.1 microcin C ABC transporter permease YejB -
PED37_RS20485 (PED37_20485) 4500891..4501910 + 1020 WP_010954919.1 ABC transporter permease -
PED37_RS20490 (PED37_20490) 4501912..4503516 + 1605 WP_010954920.1 ABC transporter ATP-binding protein -
PED37_RS20495 (PED37_20495) 4503664..4504131 + 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
PED37_RS20500 (PED37_20500) 4504128..4504703 + 576 WP_010954922.1 PIN domain-containing protein Toxin
PED37_RS20505 (PED37_20505) 4504917..4507847 + 2931 WP_049588229.1 aminotransferase -
PED37_RS20510 (PED37_20510) 4507851..4509074 - 1224 WP_010954925.1 NAD(P)/FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4484020..4507847 23827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T267239 WP_010954922.1 NZ_CP115665:4504128-4504703 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT267239 WP_049588231.1 NZ_CP115665:4503664-4504131 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References