Detailed information of TA system TA026721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2415363..2415880
• Replicon: chromosome
• Accession: NC_017349
• Organism: Staphylococcus aureus subsp. aureus LGA251

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: SARLGA251_RS12100
• Protein ID: WP_000113260.1
• Coordinates: 2415363..2415629 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SARLGA251_RS12105
• Protein ID: WP_000584499.1
• Coordinates: 2415629..2415880 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SARLGA251_RS12070	2410515..2411051	-	537	WP_000185901.1	GNAT family N-acetyltransferase	-
SARLGA251_RS12080	2411573..2412397	-	825	WP_000594634.1	formate/nitrite transporter family protein	-
SARLGA251_RS12085	2412600..2412782	-	183	WP_000079140.1	hypothetical protein	-
SARLGA251_RS12090	2412869..2413336	-	468	WP_000153340.1	SRPBCC domain-containing protein	-
SARLGA251_RS12095	2413522..2415069	-	1548	WP_000727756.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SARLGA251_RS12100	2415363..2415629	-	267	WP_000113260.1	Txe/YoeB family addiction module toxin	Toxin
SARLGA251_RS12105	2415629..2415880	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SARLGA251_RS12110	2416045..2416146	-	102	Protein_2282	hypothetical protein	-
SARLGA251_RS12115	2416153..2416752	-	600	WP_000162810.1	DsbA family protein	-
SARLGA251_RS12120	2416771..2417133	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SARLGA251_RS12125	2417388..2418638	+	1251	WP_001012239.1	FemA/FemB family glycyltransferase FmhA	-
SARLGA251_RS12130	2418735..2419466	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SARLGA251_RS12135	2419463..2420182	-	720	WP_000479579.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.99 Da
• Isoelectric Point: 10.0373

>T26721 WP_000113260.1 NC_017349:c2415629-2415363 [Staphylococcus aureus subsp. aureus LGA251]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRDGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T26721 NC_017349:c2415629-2415363 [Staphylococcus aureus subsp. aureus LGA251]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAGATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT26721 WP_000584499.1 NC_017349:c2415880-2415629 [Staphylococcus aureus subsp. aureus LGA251]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT26721 NC_017349:c2415880-2415629 [Staphylococcus aureus subsp. aureus LGA251]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: T1YB60