Detailed information of TA system TA026713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2531064..2531581
• Replicon: chromosome
• Accession: NC_017347
• Organism: Staphylococcus aureus subsp. aureus T0131

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: SAT0131_RS12945
• Protein ID: WP_000113262.1
• Coordinates: 2531064..2531330 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SAT0131_RS12950
• Protein ID: WP_000584499.1
• Coordinates: 2531330..2531581 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAT0131_RS12920	2526490..2527026	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SAT0131_RS12925	2527259..2528083	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
SAT0131_RS12930	2528300..2528482	-	183	WP_000230294.1	hypothetical protein	-
SAT0131_RS12935	2528566..2529033	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
SAT0131_RS12940	2529219..2530769	-	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAT0131_RS15825	2530958..2531029	-	72	Protein_2461	hypothetical protein	-
SAT0131_RS12945	2531064..2531330	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
SAT0131_RS12950	2531330..2531581	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAT0131_RS12955	2531746..2531847	-	102	WP_001791744.1	hypothetical protein	-
SAT0131_RS12960	2531854..2532453	-	600	WP_000162813.1	DsbA family protein	-
SAT0131_RS12965	2532472..2532834	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAT0131_RS12970	2533089..2534339	+	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
SAT0131_RS12975	2534436..2535167	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SAT0131_RS12980	2535164..2535883	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T26713 WP_000113262.1 NC_017347:c2531330-2531064 [Staphylococcus aureus subsp. aureus T0131]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T26713 NC_017347:c2531330-2531064 [Staphylococcus aureus subsp. aureus T0131]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT26713 WP_000584499.1 NC_017347:c2531581-2531330 [Staphylococcus aureus subsp. aureus T0131]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT26713 NC_017347:c2531581-2531330 [Staphylococcus aureus subsp. aureus T0131]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60