Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2039363..2040282 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag PAK35_RS09405 Protein ID WP_003900449.1
Coordinates 2039677..2040282 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag PAK35_RS09400 Protein ID WP_003410124.1
Coordinates 2039363..2039668 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS09365 (PAK35_09365) 2034824..2035201 + 378 Protein_1857 hypothetical protein -
PAK35_RS09370 (PAK35_09370) 2035198..2036238 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
PAK35_RS09375 (PAK35_09375) 2036480..2037199 + 720 WP_003410108.1 DUF433 domain-containing protein -
PAK35_RS09380 (PAK35_09380) 2037189..2037419 + 231 Protein_1860 hypothetical protein -
PAK35_RS09390 (PAK35_09390) 2038775..2038963 + 189 Protein_1862 hypothetical protein -
PAK35_RS09395 (PAK35_09395) 2038979..2039278 - 300 WP_003410120.1 hypothetical protein -
PAK35_RS09400 (PAK35_09400) 2039363..2039668 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
PAK35_RS09405 (PAK35_09405) 2039677..2040282 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PAK35_RS09410 (PAK35_09410) 2040307..2040666 - 360 WP_003410131.1 hypothetical protein -
PAK35_RS09415 (PAK35_09415) 2040826..2041479 - 654 WP_057331469.1 hypothetical protein -
PAK35_RS09420 (PAK35_09420) 2041455..2041691 - 237 WP_003410133.1 hypothetical protein -
PAK35_RS09425 (PAK35_09425) 2041898..2042794 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2033512..2047798 14286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T267114 WP_003900449.1 NZ_CP115447:c2040282-2039677 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT267114 WP_003410124.1 NZ_CP115447:c2039668-2039363 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References