Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 863478..864148 Replicon chromosome
Accession NZ_CP115447
Organism Mycobacterium tuberculosis strain BLR 9248 2019

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag PAK35_RS03955 Protein ID WP_003899954.1
Coordinates 863804..864148 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag PAK35_RS03950 Protein ID WP_003899955.1
Coordinates 863478..863807 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAK35_RS03925 (PAK35_03925) 858893..859927 + 1035 WP_003416786.1 IS30 family transposase -
PAK35_RS03930 (PAK35_03930) 860174..860383 - 210 WP_003416778.1 hypothetical protein -
PAK35_RS03935 (PAK35_03935) 860551..861816 + 1266 WP_003902423.1 hypothetical protein -
PAK35_RS03940 (PAK35_03940) 861976..862596 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
PAK35_RS03945 (PAK35_03945) 862593..862940 - 348 WP_003899956.1 DUF2384 domain-containing protein -
PAK35_RS03950 (PAK35_03950) 863478..863807 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
PAK35_RS03955 (PAK35_03955) 863804..864148 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PAK35_RS03960 (PAK35_03960) 864379..864831 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
PAK35_RS03965 (PAK35_03965) 864834..865268 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
PAK35_RS03970 (PAK35_03970) 865377..866638 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
PAK35_RS03975 (PAK35_03975) 866973..868262 - 1290 WP_003416640.1 ATP-binding protein -
PAK35_RS03980 (PAK35_03980) 868550..868843 - 294 WP_003416635.1 hypothetical protein -
PAK35_RS03985 (PAK35_03985) 868856..869067 - 212 Protein_790 (R)-hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T267098 WP_003899954.1 NZ_CP115447:c864148-863804 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT267098 WP_003899955.1 NZ_CP115447:c863807-863478 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References