Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4280213..4280907 Replicon chromosome
Accession NZ_CP114896
Organism Escherichia coli strain CM93

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6117_RS20880 Protein ID WP_001263489.1
Coordinates 4280509..4280907 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6117_RS20875 Protein ID WP_000554758.1
Coordinates 4280213..4280506 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6117_RS20855 (4275845) 4275845..4276342 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6117_RS20860 (4276566) 4276566..4278278 - 1713 Protein_4052 flagellar biosynthesis protein FlhA -
O6117_RS20865 (4278250) 4278250..4279035 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6117_RS20870 (4279106) 4279106..4280161 + 1056 WP_001226164.1 DNA polymerase IV -
O6117_RS20875 (4280213) 4280213..4280506 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6117_RS20880 (4280509) 4280509..4280907 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6117_RS20885 (4280917) 4280917..4281369 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6117_RS20890 (4281687) 4281687..4281893 + 207 Protein_4058 RtcB family protein -
O6117_RS20895 (4281889) 4281889..4282410 + 522 Protein_4059 peptide chain release factor H -
O6117_RS20900 (4282467) 4282467..4283924 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6117_RS20905 (4284185) 4284185..4284643 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4285239) 4285239..4285319 + 81 NuclAT_12 - -
- (4285239) 4285239..4285319 + 81 NuclAT_12 - -
- (4285239) 4285239..4285319 + 81 NuclAT_12 - -
- (4285239) 4285239..4285319 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T267039 WP_001263489.1 NZ_CP114896:4280509-4280907 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT267039 WP_000554758.1 NZ_CP114896:4280213-4280506 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References