Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4274169..4274710 Replicon chromosome
Accession NZ_CP114896
Organism Escherichia coli strain CM93

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag O6117_RS20840 Protein ID WP_000615983.1
Coordinates 4274169..4274447 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag O6117_RS20845 Protein ID WP_000729703.1
Coordinates 4274450..4274710 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6117_RS20825 (4271751) 4271751..4272329 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
O6117_RS20830 (4272535) 4272535..4273302 + 768 WP_012305459.1 class II glutamine amidotransferase -
O6117_RS20835 (4273273) 4273273..4274013 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
O6117_RS20840 (4274169) 4274169..4274447 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O6117_RS20845 (4274450) 4274450..4274710 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O6117_RS20850 (4274920) 4274920..4275669 + 750 WP_000056849.1 C40 family peptidase -
O6117_RS20855 (4275845) 4275845..4276342 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6117_RS20860 (4276566) 4276566..4278278 - 1713 Protein_4052 flagellar biosynthesis protein FlhA -
O6117_RS20865 (4278250) 4278250..4279035 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T267038 WP_000615983.1 NZ_CP114896:c4274447-4274169 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT267038 WP_000729703.1 NZ_CP114896:c4274710-4274450 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References