266994

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 892030..892724 Replicon chromosome
Accession NZ_CP114895
Organism Escherichia coli strain CM56

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6106_RS04285 Protein ID WP_001263489.1
Coordinates 892326..892724 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6106_RS04280 Protein ID WP_000554758.1
Coordinates 892030..892323 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6106_RS04260 (887662) 887662..888159 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6106_RS04265 (888383) 888383..890095 - 1713 Protein_810 flagellar biosynthesis protein FlhA -
O6106_RS04270 (890067) 890067..890852 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6106_RS04275 (890923) 890923..891978 + 1056 WP_001226164.1 DNA polymerase IV -
O6106_RS04280 (892030) 892030..892323 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6106_RS04285 (892326) 892326..892724 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6106_RS04290 (892734) 892734..893186 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6106_RS04295 (893504) 893504..893710 + 207 Protein_816 RtcB family protein -
O6106_RS04300 (893706) 893706..894227 + 522 Protein_817 peptide chain release factor H -
O6106_RS04305 (894284) 894284..895741 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6106_RS04310 (896002) 896002..896460 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (897056) 897056..897136 + 81 NuclAT_12 - -
- (897056) 897056..897136 + 81 NuclAT_12 - -
- (897056) 897056..897136 + 81 NuclAT_12 - -
- (897056) 897056..897136 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T266994 WP_001263489.1 NZ_CP114895:892326-892724 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT266994 WP_000554758.1 NZ_CP114895:892030-892323 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References