Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4181534..4182228 Replicon chromosome
Accession NZ_CP114894
Organism Escherichia coli strain CM19

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6X69_RS20430 Protein ID WP_001263489.1
Coordinates 4181830..4182228 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6X69_RS20425 Protein ID WP_000554758.1
Coordinates 4181534..4181827 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6X69_RS20405 (4177166) 4177166..4177663 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6X69_RS20410 (4177887) 4177887..4179599 - 1713 Protein_3962 flagellar biosynthesis protein FlhA -
O6X69_RS20415 (4179571) 4179571..4180356 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6X69_RS20420 (4180427) 4180427..4181482 + 1056 WP_001226164.1 DNA polymerase IV -
O6X69_RS20425 (4181534) 4181534..4181827 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6X69_RS20430 (4181830) 4181830..4182228 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6X69_RS20435 (4182238) 4182238..4182690 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6X69_RS20440 (4183008) 4183008..4183214 + 207 Protein_3968 RtcB family protein -
O6X69_RS20445 (4183210) 4183210..4183731 + 522 Protein_3969 peptide chain release factor H -
O6X69_RS20450 (4183788) 4183788..4185245 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6X69_RS20455 (4185506) 4185506..4185964 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4186560) 4186560..4186640 + 81 NuclAT_12 - -
- (4186560) 4186560..4186640 + 81 NuclAT_12 - -
- (4186560) 4186560..4186640 + 81 NuclAT_12 - -
- (4186560) 4186560..4186640 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T266987 WP_001263489.1 NZ_CP114894:4181830-4182228 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT266987 WP_000554758.1 NZ_CP114894:4181534-4181827 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References