Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4175490..4176031 Replicon chromosome
Accession NZ_CP114894
Organism Escherichia coli strain CM19

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag O6X69_RS20390 Protein ID WP_000615983.1
Coordinates 4175490..4175768 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag O6X69_RS20395 Protein ID WP_000729703.1
Coordinates 4175771..4176031 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6X69_RS20375 (4173072) 4173072..4173650 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
O6X69_RS20380 (4173856) 4173856..4174623 + 768 WP_012305459.1 class II glutamine amidotransferase -
O6X69_RS20385 (4174594) 4174594..4175334 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
O6X69_RS20390 (4175490) 4175490..4175768 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O6X69_RS20395 (4175771) 4175771..4176031 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O6X69_RS20400 (4176241) 4176241..4176990 + 750 WP_000056849.1 C40 family peptidase -
O6X69_RS20405 (4177166) 4177166..4177663 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6X69_RS20410 (4177887) 4177887..4179599 - 1713 Protein_3962 flagellar biosynthesis protein FlhA -
O6X69_RS20415 (4179571) 4179571..4180356 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T266986 WP_000615983.1 NZ_CP114894:c4175768-4175490 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT266986 WP_000729703.1 NZ_CP114894:c4176031-4175771 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References