Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 292542..293083 Replicon chromosome
Accession NZ_CP114893
Organism Escherichia coli strain CM13

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag O6R07_RS01535 Protein ID WP_000615983.1
Coordinates 292805..293083 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag O6R07_RS01530 Protein ID WP_000729703.1
Coordinates 292542..292802 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6R07_RS01510 (288217) 288217..289002 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6R07_RS01515 (288974) 288974..290686 + 1713 Protein_299 flagellar biosynthesis protein FlhA -
O6R07_RS01520 (290910) 290910..291407 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6R07_RS01525 (291583) 291583..292332 - 750 WP_000056849.1 C40 family peptidase -
O6R07_RS01530 (292542) 292542..292802 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O6R07_RS01535 (292805) 292805..293083 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O6R07_RS01540 (293239) 293239..293979 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
O6R07_RS01545 (293950) 293950..294717 - 768 WP_012305459.1 class II glutamine amidotransferase -
O6R07_RS01550 (294923) 294923..295501 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T266944 WP_000615983.1 NZ_CP114893:292805-293083 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT266944 WP_000729703.1 NZ_CP114893:292542-292802 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References