Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 286345..287039 Replicon chromosome
Accession NZ_CP114893
Organism Escherichia coli strain CM13

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6R07_RS01495 Protein ID WP_001263489.1
Coordinates 286345..286743 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6R07_RS01500 Protein ID WP_000554758.1
Coordinates 286746..287039 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (281933) 281933..282013 - 81 NuclAT_12 - -
- (281933) 281933..282013 - 81 NuclAT_12 - -
- (281933) 281933..282013 - 81 NuclAT_12 - -
- (281933) 281933..282013 - 81 NuclAT_12 - -
O6R07_RS01470 (282609) 282609..283067 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
O6R07_RS01475 (283328) 283328..284785 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6R07_RS01480 (284842) 284842..285363 - 522 Protein_292 peptide chain release factor H -
O6R07_RS01485 (285359) 285359..285565 - 207 Protein_293 RtcB family protein -
O6R07_RS01490 (285883) 285883..286335 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6R07_RS01495 (286345) 286345..286743 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6R07_RS01500 (286746) 286746..287039 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6R07_RS01505 (287091) 287091..288146 - 1056 WP_001226164.1 DNA polymerase IV -
O6R07_RS01510 (288217) 288217..289002 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6R07_RS01515 (288974) 288974..290686 + 1713 Protein_299 flagellar biosynthesis protein FlhA -
O6R07_RS01520 (290910) 290910..291407 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T266943 WP_001263489.1 NZ_CP114893:c286743-286345 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT266943 WP_000554758.1 NZ_CP114893:c287039-286746 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References