Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4047805..4048499 Replicon chromosome
Accession NZ_CP114892
Organism Escherichia coli strain CM5

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6Q95_RS19770 Protein ID WP_001263489.1
Coordinates 4048101..4048499 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6Q95_RS19765 Protein ID WP_000554758.1
Coordinates 4047805..4048098 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6Q95_RS19745 (4043437) 4043437..4043934 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6Q95_RS19750 (4044158) 4044158..4045870 - 1713 Protein_3845 flagellar biosynthesis protein FlhA -
O6Q95_RS19755 (4045842) 4045842..4046627 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6Q95_RS19760 (4046698) 4046698..4047753 + 1056 WP_001226164.1 DNA polymerase IV -
O6Q95_RS19765 (4047805) 4047805..4048098 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6Q95_RS19770 (4048101) 4048101..4048499 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6Q95_RS19775 (4048509) 4048509..4048961 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6Q95_RS19780 (4049279) 4049279..4049485 + 207 Protein_3851 RtcB family protein -
O6Q95_RS19785 (4049481) 4049481..4050002 + 522 Protein_3852 peptide chain release factor H -
O6Q95_RS19790 (4050059) 4050059..4051516 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6Q95_RS19795 (4051777) 4051777..4052235 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4052831) 4052831..4052911 + 81 NuclAT_12 - -
- (4052831) 4052831..4052911 + 81 NuclAT_12 - -
- (4052831) 4052831..4052911 + 81 NuclAT_12 - -
- (4052831) 4052831..4052911 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T266938 WP_001263489.1 NZ_CP114892:4048101-4048499 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT266938 WP_000554758.1 NZ_CP114892:4047805-4048098 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References