Detailed information of TA system TA026693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2388887..2389404
• Replicon: chromosome
• Accession: NC_017343
• Organism: Staphylococcus aureus subsp. aureus ECT-R 2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: ECTR2_RS12035
• Protein ID: WP_000113265.1
• Coordinates: 2388887..2389153 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: ECTR2_RS12040
• Protein ID: WP_000584499.1
• Coordinates: 2389153..2389404 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTR2_RS12010	2384315..2384851	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
ECTR2_RS12015	2385085..2385909	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
ECTR2_RS12020	2386126..2386308	-	183	WP_000230292.1	hypothetical protein	-
ECTR2_RS12025	2386392..2386859	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
ECTR2_RS12030	2387045..2388592	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
ECTR2_RS14430	2388781..2388852	-	72	Protein_2276	hypothetical protein	-
ECTR2_RS12035	2388887..2389153	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
ECTR2_RS12040	2389153..2389404	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECTR2_RS12045	2389569..2389670	-	102	Protein_2279	hypothetical protein	-
ECTR2_RS12050	2389677..2390276	-	600	WP_000162810.1	DsbA family protein	-
ECTR2_RS12055	2390295..2390657	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
ECTR2_RS12060	2390912..2392162	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
ECTR2_RS12065	2392259..2392990	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
ECTR2_RS12070	2392987..2393706	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T26693 WP_000113265.1 NC_017343:c2389153-2388887 [Staphylococcus aureus subsp. aureus ECT-R 2]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T26693 NC_017343:c2389153-2388887 [Staphylococcus aureus subsp. aureus ECT-R 2]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT26693 WP_000584499.1 NC_017343:c2389404-2389153 [Staphylococcus aureus subsp. aureus ECT-R 2]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT26693 NC_017343:c2389404-2389153 [Staphylococcus aureus subsp. aureus ECT-R 2]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60