Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3126157..3126698 Replicon chromosome
Accession NZ_CP114891
Organism Escherichia coli strain CM2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag O6Q44_RS15480 Protein ID WP_000615983.1
Coordinates 3126420..3126698 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag O6Q44_RS15475 Protein ID WP_000729703.1
Coordinates 3126157..3126417 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6Q44_RS15455 (3121832) 3121832..3122617 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6Q44_RS15460 (3122589) 3122589..3124301 + 1713 Protein_3020 flagellar biosynthesis protein FlhA -
O6Q44_RS15465 (3124525) 3124525..3125022 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
O6Q44_RS15470 (3125198) 3125198..3125947 - 750 WP_000056849.1 C40 family peptidase -
O6Q44_RS15475 (3126157) 3126157..3126417 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O6Q44_RS15480 (3126420) 3126420..3126698 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O6Q44_RS15485 (3126854) 3126854..3127594 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
O6Q44_RS15490 (3127565) 3127565..3128332 - 768 WP_012305459.1 class II glutamine amidotransferase -
O6Q44_RS15495 (3128538) 3128538..3129116 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T266911 WP_000615983.1 NZ_CP114891:3126420-3126698 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT266911 WP_000729703.1 NZ_CP114891:3126157-3126417 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References