Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3119960..3120654 Replicon chromosome
Accession NZ_CP114891
Organism Escherichia coli strain CM2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag O6Q44_RS15440 Protein ID WP_001263489.1
Coordinates 3119960..3120358 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag O6Q44_RS15445 Protein ID WP_000554758.1
Coordinates 3120361..3120654 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3115548) 3115548..3115628 - 81 NuclAT_12 - -
- (3115548) 3115548..3115628 - 81 NuclAT_12 - -
- (3115548) 3115548..3115628 - 81 NuclAT_12 - -
- (3115548) 3115548..3115628 - 81 NuclAT_12 - -
O6Q44_RS15415 (3116224) 3116224..3116682 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
O6Q44_RS15420 (3116943) 3116943..3118400 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
O6Q44_RS15425 (3118457) 3118457..3118978 - 522 Protein_3013 peptide chain release factor H -
O6Q44_RS15430 (3118974) 3118974..3119180 - 207 Protein_3014 RtcB family protein -
O6Q44_RS15435 (3119498) 3119498..3119950 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
O6Q44_RS15440 (3119960) 3119960..3120358 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
O6Q44_RS15445 (3120361) 3120361..3120654 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
O6Q44_RS15450 (3120706) 3120706..3121761 - 1056 WP_001226164.1 DNA polymerase IV -
O6Q44_RS15455 (3121832) 3121832..3122617 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
O6Q44_RS15460 (3122589) 3122589..3124301 + 1713 Protein_3020 flagellar biosynthesis protein FlhA -
O6Q44_RS15465 (3124525) 3124525..3125022 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T266910 WP_001263489.1 NZ_CP114891:c3120358-3119960 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT266910 WP_000554758.1 NZ_CP114891:c3120654-3120361 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References