Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4580422..4581024 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag O3296_RS21975 Protein ID WP_000897305.1
Coordinates 4580713..4581024 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag O3296_RS21970 Protein ID WP_000356397.1
Coordinates 4580422..4580712 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS21945 (4575854) 4575854..4576489 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
O3296_RS21950 (4576486) 4576486..4577415 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
O3296_RS21955 (4577928) 4577928..4578908 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
O3296_RS21960 (4579138) 4579138..4579380 - 243 WP_001086388.1 protein YiiF -
O3296_RS21965 (4579599) 4579599..4579817 - 219 WP_001295676.1 CopG family transcriptional regulator -
O3296_RS21970 (4580422) 4580422..4580712 - 291 WP_000356397.1 NadS family protein Antitoxin
O3296_RS21975 (4580713) 4580713..4581024 - 312 WP_000897305.1 hypothetical protein Toxin
O3296_RS21980 (4581253) 4581253..4582161 + 909 WP_001162704.1 alpha/beta hydrolase -
O3296_RS21985 (4582225) 4582225..4583166 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
O3296_RS21990 (4583211) 4583211..4583648 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
O3296_RS21995 (4583645) 4583645..4584517 - 873 WP_000920762.1 virulence factor BrkB family protein -
O3296_RS22000 (4584511) 4584511..4585110 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4577928..4578908 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T266776 WP_000897305.1 NZ_CP114592:c4581024-4580713 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT266776 WP_000356397.1 NZ_CP114592:c4580712-4580422 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References