Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3740961..3741502 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag O3296_RS18190 Protein ID WP_000615976.1
Coordinates 3741224..3741502 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag O3296_RS18185 Protein ID WP_000729704.1
Coordinates 3740961..3741221 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS18160 (3736744) 3736744..3737529 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
O3296_RS18165 (3737501) 3737501..3739213 + 1713 Protein_3558 flagellar biosynthesis protein FlhA -
O3296_RS18170 (3739318) 3739318..3739596 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
O3296_RS18175 (3739589) 3739589..3739945 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
O3296_RS18180 (3740002) 3740002..3740775 - 774 WP_000543896.1 C40 family peptidase -
O3296_RS18185 (3740961) 3740961..3741221 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O3296_RS18190 (3741224) 3741224..3741502 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O3296_RS18195 (3741658) 3741658..3742398 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
O3296_RS18200 (3742369) 3742369..3743136 - 768 WP_000333380.1 class II glutamine amidotransferase -
O3296_RS18205 (3743342) 3743342..3743920 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T266772 WP_000615976.1 NZ_CP114592:3741224-3741502 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT266772 WP_000729704.1 NZ_CP114592:3740961-3741221 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References