Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1052055..1052782 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag O3296_RS05085 Protein ID WP_000547563.1
Coordinates 1052055..1052366 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag O3296_RS05090 Protein ID WP_000126294.1
Coordinates 1052363..1052782 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS05055 (1047198) 1047198..1048907 + 1710 WP_063625439.1 formate hydrogenlyase subunit HycE -
O3296_RS05060 (1048917) 1048917..1049459 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
O3296_RS05065 (1049459) 1049459..1050226 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
O3296_RS05070 (1050223) 1050223..1050633 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
O3296_RS05075 (1050626) 1050626..1051096 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
O3296_RS05080 (1051139) 1051139..1051882 + 744 WP_058101263.1 hypothetical protein -
O3296_RS05085 (1052055) 1052055..1052366 + 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
O3296_RS05090 (1052363) 1052363..1052782 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
O3296_RS05095 (1052896) 1052896..1054320 - 1425 WP_000110365.1 6-phospho-beta-glucosidase AscB -
O3296_RS05100 (1054329) 1054329..1055786 - 1458 WP_001107882.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
O3296_RS05105 (1056046) 1056046..1057056 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
O3296_RS05110 (1057205) 1057205..1057732 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T266761 WP_000547563.1 NZ_CP114592:1052055-1052366 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT266761 WP_000126294.1 NZ_CP114592:1052363-1052782 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References