Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 605824..606623 Replicon chromosome
Accession NZ_CP114592
Organism Escherichia coli strain WEC

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag O3296_RS02960 Protein ID WP_000347267.1
Coordinates 605824..606288 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag O3296_RS02965 Protein ID WP_001307405.1
Coordinates 606288..606623 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3296_RS02930 (600825) 600825..601259 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
O3296_RS02935 (601277) 601277..602155 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
O3296_RS02940 (602145) 602145..602924 - 780 WP_063625504.1 PTS N-acetylgalactosamine transporter subunit IIC -
O3296_RS02945 (602935) 602935..603408 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
O3296_RS02950 (603431) 603431..604711 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
O3296_RS02955 (604960) 604960..605769 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
O3296_RS02960 (605824) 605824..606288 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
O3296_RS02965 (606288) 606288..606623 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
O3296_RS02970 (606772) 606772..608343 - 1572 WP_001273741.1 galactarate dehydratase -
O3296_RS02975 (608718) 608718..610052 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
O3296_RS02980 (610068) 610068..610838 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 605824..618766 12942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T266758 WP_000347267.1 NZ_CP114592:c606288-605824 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT266758 WP_001307405.1 NZ_CP114592:c606623-606288 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References