Detailed information of TA system TA026653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2563608..2564125
• Replicon: chromosome
• Accession: NC_017341
• Organism: Staphylococcus aureus subsp. aureus str. JKD6008

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: SAA6008_RS13335
• Protein ID: WP_000113262.1
• Coordinates: 2563608..2563874 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SAA6008_RS13340
• Protein ID: WP_000584499.1
• Coordinates: 2563874..2564125 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAA6008_RS13310	2559034..2559570	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SAA6008_RS13315	2559803..2560627	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
SAA6008_RS13320	2560844..2561026	-	183	WP_000230294.1	hypothetical protein	-
SAA6008_RS13325	2561110..2561577	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
SAA6008_RS13330	2561763..2563313	-	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAA6008_RS16140	2563502..2563573	-	72	Protein_2513	hypothetical protein	-
SAA6008_RS13335	2563608..2563874	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
SAA6008_RS13340	2563874..2564125	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAA6008_RS13345	2564290..2564391	-	102	WP_001791744.1	hypothetical protein	-
SAA6008_RS13350	2564398..2564997	-	600	WP_000162813.1	DsbA family protein	-
SAA6008_RS13355	2565016..2565378	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAA6008_RS13360	2565633..2566883	+	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
SAA6008_RS13365	2566980..2567711	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SAA6008_RS13370	2567708..2568427	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T26653 WP_000113262.1 NC_017341:c2563874-2563608 [Staphylococcus aureus subsp. aureus str. JKD6008]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T26653 NC_017341:c2563874-2563608 [Staphylococcus aureus subsp. aureus str. JKD6008]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT26653 WP_000584499.1 NC_017341:c2564125-2563874 [Staphylococcus aureus subsp. aureus str. JKD6008]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT26653 NC_017341:c2564125-2563874 [Staphylococcus aureus subsp. aureus str. JKD6008]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60