Detailed information of TA system TA266380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4167024..4167621
• Replicon: chromosome
• Accession: NZ_CP114164
• Organism: Klebsiella variicola strain 2022CK-00564

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: OIX91_RS20285
• Protein ID: WP_012542526.1
• Coordinates: 4167304..4167621 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OIX91_RS20280
• Protein ID: WP_012542525.1
• Coordinates: 4167024..4167311 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OIX91_RS20250 (OIX91_20250)	4162928..4163176	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
OIX91_RS20255 (OIX91_20255)	4163193..4163534	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
OIX91_RS20260 (OIX91_20260)	4163565..4164680	-	1116	WP_162823038.1	MBL fold metallo-hydrolase	-
OIX91_RS20265 (OIX91_20265)	4164860..4165441	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
OIX91_RS20270 (OIX91_20270)	4165441..4165809	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
OIX91_RS20275 (OIX91_20275)	4165929..4166582	+	654	WP_032729541.1	oxygen-insensitive NAD(P)H nitroreductase	-
OIX91_RS20280 (OIX91_20280)	4167024..4167311	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
OIX91_RS20285 (OIX91_20285)	4167304..4167621	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OIX91_RS20290 (OIX91_20290)	4167806..4168849	-	1044	WP_269136554.1	DUF2157 domain-containing protein	-
OIX91_RS20295 (OIX91_20295)	4169314..4170180	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
OIX91_RS20300 (OIX91_20300)	4170289..4171716	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12092.35 Da
• Isoelectric Point: 11.2767

>T266380 WP_012542526.1 NZ_CP114164:c4167621-4167304 [Klebsiella variicola]
IFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T266380 NZ_CP114164:c4167621-4167304 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT266380 WP_012542525.1 NZ_CP114164:c4167311-4167024 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT266380 NZ_CP114164:c4167311-4167024 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2