Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4014294..4014988 Replicon chromosome
Accession NZ_CP114130
Organism Escherichia coli strain NJS001

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag N0F11_RS20255 Protein ID WP_001263495.1
Coordinates 4014294..4014692 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N0F11_RS20260 Protein ID WP_000554757.1
Coordinates 4014695..4014988 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4009881) 4009881..4009961 - 81 NuclAT_9 - -
- (4009881) 4009881..4009961 - 81 NuclAT_9 - -
- (4009881) 4009881..4009961 - 81 NuclAT_9 - -
- (4009881) 4009881..4009961 - 81 NuclAT_9 - -
N0F11_RS20230 (4010557) 4010557..4011015 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N0F11_RS20235 (4011276) 4011276..4012733 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
N0F11_RS20240 (4012790) 4012790..4013311 - 522 Protein_3971 peptide chain release factor H -
N0F11_RS20245 (4013310) 4013310..4013513 - 204 Protein_3972 RtcB family protein -
N0F11_RS20250 (4013832) 4013832..4014284 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
N0F11_RS20255 (4014294) 4014294..4014692 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N0F11_RS20260 (4014695) 4014695..4014988 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N0F11_RS20265 (4015040) 4015040..4016095 - 1056 WP_001226186.1 DNA polymerase IV -
N0F11_RS20270 (4016166) 4016166..4016951 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
N0F11_RS20275 (4016923) 4016923..4018629 + 1707 Protein_3978 flagellar biosynthesis protein FlhA -
N0F11_RS20280 (4018780) 4018780..4019277 - 498 Protein_3979 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T266310 WP_001263495.1 NZ_CP114130:c4014692-4014294 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT266310 WP_000554757.1 NZ_CP114130:c4014988-4014695 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References