Detailed information of TA system TA266298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2217126..2217497
• Replicon: chromosome
• Accession: NZ_CP114130
• Organism: Escherichia coli strain NJS001

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: N0F11_RS10995
• Protein ID: WP_042853000.1
• Coordinates: 2217303..2217497 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2217126..2217304 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N0F11_RS10965 (2212878)	2212878..2213048	+	171	WP_001625136.1	protein YnaL	-
N0F11_RS10970 (2213081)	2213081..2214454	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
N0F11_RS10975 (2214583)	2214583..2215518	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
N0F11_RS10980 (2215570)	2215570..2216805	-	1236	WP_000040845.1	site-specific integrase	-
N0F11_RS10985 (2216807)	2216807..2217022	-	216	WP_000079604.1	excisionase XisR	-
- (2217126)	2217126..2217304	+	179	NuclAT_0	-	Antitoxin
- (2217126)	2217126..2217304	+	179	NuclAT_0	-	Antitoxin
- (2217126)	2217126..2217304	+	179	NuclAT_0	-	Antitoxin
- (2217126)	2217126..2217304	+	179	NuclAT_0	-	Antitoxin
N0F11_RS10990 (2217122)	2217122..2217310	-	189	WP_001356607.1	DUF1187 family protein	-
N0F11_RS10995 (2217303)	2217303..2217497	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
N0F11_RS11000 (2217554)	2217554..2218363	-	810	WP_000166315.1	recombination protein RecT	-
N0F11_RS11005 (2218356)	2218356..2220983	-	2628	WP_032324100.1	exodeoxyribonuclease VIII	-
N0F11_RS11010 (2221064)	2221064..2221234	-	171	WP_001427414.1	YdaE family protein	-
N0F11_RS11015 (2221234)	2221234..2221455	-	222	WP_000560218.1	killing protein KilR	-
N0F11_RS11020 (2221876)	2221876..2222028	-	153	WP_001169150.1	DUF1391 family protein	-
N0F11_RS11025 (2222039)	2222039..2222173	-	135	WP_000233806.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2215570..2256470	40900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T266298 WP_042853000.1 NZ_CP114130:c2217497-2217303 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

Antitoxin

Nucleotide

• Length: 179 bp

>AT266298 NZ_CP114130:2217126-2217304 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin