Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3878479..3879173 Replicon chromosome
Accession NZ_CP113503
Organism Escherichia coli strain 16843

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag OWO50_RS18620 Protein ID WP_001263500.1
Coordinates 3878479..3878877 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OWO50_RS18625 Protein ID WP_000554758.1
Coordinates 3878880..3879173 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OWO50_RS18595 (3873844) 3873844..3874302 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
OWO50_RS18600 (3874563) 3874563..3876020 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
OWO50_RS18605 (3876077) 3876077..3876691 - 615 WP_000602124.1 peptide chain release factor H -
OWO50_RS18610 (3876688) 3876688..3877827 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
OWO50_RS18615 (3878017) 3878017..3878469 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
OWO50_RS18620 (3878479) 3878479..3878877 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OWO50_RS18625 (3878880) 3878880..3879173 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OWO50_RS18630 (3879225) 3879225..3880280 - 1056 WP_001226168.1 DNA polymerase IV -
OWO50_RS18635 (3880351) 3880351..3881136 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
OWO50_RS18640 (3881108) 3881108..3882820 + 1713 Protein_3659 flagellar biosynthesis protein FlhA -
OWO50_RS18645 (3882848) 3882848..3883057 + 210 WP_071778446.1 hypothetical protein -
OWO50_RS18650 (3883140) 3883140..3883637 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3878479..3898833 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T265829 WP_001263500.1 NZ_CP113503:c3878877-3878479 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT265829 WP_000554758.1 NZ_CP113503:c3879173-3878880 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References