Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3572140..3572819 Replicon chromosome
Accession NZ_CP113503
Organism Escherichia coli strain 16843

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag OWO50_RS17190 Protein ID WP_000057523.1
Coordinates 3572517..3572819 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag OWO50_RS17185 Protein ID WP_000806442.1
Coordinates 3572140..3572481 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OWO50_RS17175 (3568384) 3568384..3569316 - 933 WP_000883025.1 glutaminase A -
OWO50_RS17180 (3569578) 3569578..3572082 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
OWO50_RS17185 (3572140) 3572140..3572481 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
OWO50_RS17190 (3572517) 3572517..3572819 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OWO50_RS17195 (3572952) 3572952..3573746 + 795 WP_000365148.1 TraB/GumN family protein -
OWO50_RS17200 (3573950) 3573950..3574429 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
OWO50_RS17205 (3574453) 3574453..3575253 + 801 WP_000439798.1 hypothetical protein -
OWO50_RS17210 (3575250) 3575250..3575753 + 504 WP_000667000.1 hypothetical protein -
OWO50_RS17215 (3575790) 3575790..3577442 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T265826 WP_000057523.1 NZ_CP113503:c3572819-3572517 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT265826 WP_000806442.1 NZ_CP113503:c3572481-3572140 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References