Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5013701..5014303 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name higB Uniprot ID A0A4Y9BEM6
Locus tag E5R32_RS24220 Protein ID WP_059332311.1
Coordinates 5013992..5014303 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E5R32_RS24215 Protein ID WP_000356395.1
Coordinates 5013701..5013991 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS24180 (5009325) 5009325..5010227 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
E5R32_RS24185 (5010224) 5010224..5010859 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
E5R32_RS24190 (5010856) 5010856..5011785 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
E5R32_RS24195 (5011967) 5011967..5012209 - 243 WP_001309881.1 CopG family transcriptional regulator -
E5R32_RS24200 (5012428) 5012428..5012646 - 219 WP_001295676.1 CopG family transcriptional regulator -
E5R32_RS24205 (5013065) 5013065..5013343 - 279 WP_001315112.1 hypothetical protein -
E5R32_RS24210 (5013395) 5013395..5013616 - 222 WP_001550354.1 hypothetical protein -
E5R32_RS24215 (5013701) 5013701..5013991 - 291 WP_000356395.1 NadS family protein Antitoxin
E5R32_RS24220 (5013992) 5013992..5014303 - 312 WP_059332311.1 hypothetical protein Toxin
E5R32_RS24225 (5014532) 5014532..5015440 + 909 WP_001550353.1 alpha/beta hydrolase -
E5R32_RS24230 (5015608) 5015608..5016522 - 915 WP_109553727.1 transposase -
E5R32_RS24235 (5016535) 5016535..5017422 - 888 Protein_4736 hypothetical protein -
E5R32_RS24240 (5017838) 5017838..5018779 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
E5R32_RS24245 (5018824) 5018824..5019261 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12212.25 Da        Isoelectric Point: 10.0241

>T265801 WP_059332311.1 NZ_CP113493:c5014303-5013992 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPHYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT265801 WP_000356395.1 NZ_CP113493:c5013991-5013701 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y9BEM6

Antitoxin

Source ID Structure

References