Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4285134..4285861 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag E5R32_RS20750 Protein ID WP_000547555.1
Coordinates 4285550..4285861 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E5R32_RS20745 Protein ID WP_000126294.1
Coordinates 4285134..4285553 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS20725 (4280219) 4280219..4280746 - 528 WP_001078777.1 electron transport protein HydN -
E5R32_RS20730 (4280895) 4280895..4281905 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
E5R32_RS20735 (4282165) 4282165..4283622 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
E5R32_RS20740 (4283631) 4283631..4285055 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
E5R32_RS20745 (4285134) 4285134..4285553 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
E5R32_RS20750 (4285550) 4285550..4285861 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
E5R32_RS20755 (4286028) 4286028..4286498 - 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
E5R32_RS20760 (4286491) 4286491..4286901 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
E5R32_RS20765 (4286898) 4286898..4287665 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
E5R32_RS20770 (4287665) 4287665..4288207 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
E5R32_RS20775 (4288217) 4288217..4289926 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T265795 WP_000547555.1 NZ_CP113493:c4285861-4285550 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT265795 WP_000126294.1 NZ_CP113493:c4285553-4285134 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References