Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629674..630473 Replicon chromosome
Accession NZ_CP113493
Organism Escherichia coli strain GTEN_97

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag E5R32_RS03030 Protein ID WP_000347270.1
Coordinates 629674..630138 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag E5R32_RS03035 Protein ID WP_001551693.1
Coordinates 630138..630473 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5R32_RS03000 (624675) 624675..625109 - 435 WP_268101593.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
E5R32_RS03005 (625127) 625127..626005 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
E5R32_RS03010 (625995) 625995..626774 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
E5R32_RS03015 (626785) 626785..627258 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
E5R32_RS03020 (627281) 627281..628561 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
E5R32_RS03025 (628810) 628810..629619 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
E5R32_RS03030 (629674) 629674..630138 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
E5R32_RS03035 (630138) 630138..630473 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
E5R32_RS03040 (630622) 630622..632193 - 1572 WP_001273738.1 galactarate dehydratase -
E5R32_RS03045 (632568) 632568..633902 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
E5R32_RS03050 (633918) 633918..634688 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T265778 WP_000347270.1 NZ_CP113493:c630138-629674 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT265778 WP_001551693.1 NZ_CP113493:c630473-630138 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References