Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1541226..1541953 Replicon chromosome
Accession NZ_CP113486
Organism Escherichia coli strain GTEN_23

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag FG550_RS07405 Protein ID WP_000547555.1
Coordinates 1541642..1541953 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FG550_RS07400 Protein ID WP_000126294.1
Coordinates 1541226..1541645 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG550_RS07380 (1536311) 1536311..1536838 - 528 WP_001078777.1 electron transport protein HydN -
FG550_RS07385 (1536987) 1536987..1537997 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
FG550_RS07390 (1538257) 1538257..1539714 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
FG550_RS07395 (1539723) 1539723..1541147 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
FG550_RS07400 (1541226) 1541226..1541645 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
FG550_RS07405 (1541642) 1541642..1541953 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
FG550_RS07410 (1542120) 1542120..1542590 - 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
FG550_RS07415 (1542583) 1542583..1542993 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
FG550_RS07420 (1542990) 1542990..1543757 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
FG550_RS07425 (1543757) 1543757..1544299 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
FG550_RS07430 (1544309) 1544309..1546018 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T265747 WP_000547555.1 NZ_CP113486:c1541953-1541642 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT265747 WP_000126294.1 NZ_CP113486:c1541645-1541226 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References