Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1317210..1317807 Replicon chromosome
Accession NZ_CP113191
Organism Klebsiella pneumoniae strain SYCC2

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag OT482_RS09320 Protein ID WP_004142563.1
Coordinates 1317210..1317527 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag OT482_RS09325 Protein ID WP_004142561.1
Coordinates 1317520..1317807 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT482_RS09305 (1312910) 1312910..1314337 - 1428 WP_009308097.1 MFS transporter -
OT482_RS09310 (1314446) 1314446..1315312 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
OT482_RS09315 (1315982) 1315982..1317025 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
OT482_RS09320 (1317210) 1317210..1317527 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OT482_RS09325 (1317520) 1317520..1317807 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
OT482_RS09330 (1318072) 1318072..1318725 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
OT482_RS09335 (1318845) 1318845..1319213 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
OT482_RS09340 (1319213) 1319213..1319794 - 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
OT482_RS09345 (1319974) 1319974..1321089 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
OT482_RS09350 (1321120) 1321120..1321461 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
OT482_RS09355 (1321479) 1321479..1321727 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T265531 WP_004142563.1 NZ_CP113191:1317210-1317527 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT265531 WP_004142561.1 NZ_CP113191:1317520-1317807 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References