Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 88326..89053 Replicon plasmid pTSNTC10-1_tmex_202k
Accession NZ_CP113166
Organism Klebsiella pneumoniae strain TSNTC10-1

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag OT484_RS27395 Protein ID WP_011251285.1
Coordinates 88326..88637 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag OT484_RS27400 Protein ID WP_011251286.1
Coordinates 88634..89053 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT484_RS27370 (OT484_27370) 85092..85271 - 180 Protein_89 IS5/IS1182 family transposase -
OT484_RS27375 (OT484_27375) 85311..86839 - 1529 Protein_90 IS66 family transposase -
OT484_RS27380 (OT484_27380) 86888..87235 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
OT484_RS27385 (OT484_27385) 87232..87570 - 339 WP_023288266.1 transposase -
OT484_RS27390 (OT484_27390) 87684..88121 + 438 Protein_93 DDE-type integrase/transposase/recombinase -
OT484_RS27395 (OT484_27395) 88326..88637 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
OT484_RS27400 (OT484_27400) 88634..89053 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
OT484_RS27405 (OT484_27405) 89200..90168 + 969 WP_077268729.1 IS5 family transposase -
OT484_RS27410 (OT484_27410) 90237..90772 - 536 Protein_97 transposase -
OT484_RS27415 (OT484_27415) 91297..92664 - 1368 WP_032437385.1 formimidoylglutamate deiminase -
OT484_RS27420 (OT484_27420) 92767..93528 + 762 WP_017900871.1 histidine utilization repressor -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / blaDHA-1 / qnrB4 / tet(A) / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / aac(3)-IId / mph(A) / qnrB91 - 1..202189 202189
- inside IScluster/Tn - - 80402..90168 9766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T265461 WP_011251285.1 NZ_CP113166:88326-88637 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT265461 WP_011251286.1 NZ_CP113166:88634-89053 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References