Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 434485..435103 Replicon chromosome
Accession NC_017328
Organism Shigella flexneri 2002017

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag SFXV_RS02330 Protein ID WP_001291435.1
Coordinates 434485..434703 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag SFXV_RS02335 Protein ID WP_000344797.1
Coordinates 434729..435103 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFXV_RS02305 (430377) 430377..430688 - 312 WP_000409911.1 MGMT family protein -
SFXV_RS02310 (431067) 431067..431420 + 354 WP_000878140.1 DUF1428 domain-containing protein -
SFXV_RS02315 (431462) 431462..433012 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
SFXV_RS02320 (433176) 433176..433646 - 471 WP_000136192.1 YlaC family protein -
SFXV_RS02325 (433762) 433762..434313 - 552 Protein_438 maltose O-acetyltransferase -
SFXV_RS02330 (434485) 434485..434703 - 219 WP_001291435.1 HHA domain-containing protein Toxin
SFXV_RS02335 (434729) 434729..435103 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
SFXV_RS02340 (435649) 435649..438798 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
SFXV_RS02345 (438821) 438821..440014 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 429019..430347 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T26533 WP_001291435.1 NC_017328:c434703-434485 [Shigella flexneri 2002017]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T26533 NC_017328:c434703-434485 [Shigella flexneri 2002017]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT26533 WP_000344797.1 NC_017328:c435103-434729 [Shigella flexneri 2002017]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT26533 NC_017328:c435103-434729 [Shigella flexneri 2002017]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References