Detailed information of TA system TA026532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 397303..398147
• Replicon: chromosome
• Accession: NC_017328
• Organism: Shigella flexneri 2002017

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: SFXV_RS02135
• Protein ID: WP_014532172.1
• Coordinates: 397303..397764 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: SFXV_RS02145
• Protein ID: WP_005053053.1
• Coordinates: 397836..398147 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFXV_RS02115 (392471)	392471..394201	-	1731	WP_000645013.1	hypothetical protein	-
SFXV_RS02120 (394254)	394254..396407	-	2154	WP_005060811.1	SEL1-like repeat protein	-
SFXV_RS02130 (396558)	396558..397255	+	698	WP_223368647.1	IS1 family transposase	-
SFXV_RS02135 (397303)	397303..397764	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
SFXV_RS02145 (397836)	397836..398147	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
SFXV_RS02150 (398331)	398331..399221	-	891	WP_000971346.1	heme o synthase	-
SFXV_RS02155 (399233)	399233..399562	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
SFXV_RS02160 (399562)	399562..400176	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
SFXV_RS02165 (400166)	400166..402157	-	1992	Protein_406	cytochrome o ubiquinol oxidase subunit I	-
SFXV_RS02170 (402179)	402179..402676	-	498	Protein_407	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	396752..397255	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T26532 WP_014532172.1 NC_017328:c397764-397303 [Shigella flexneri 2002017]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T26532 NC_017328:c397764-397303 [Shigella flexneri 2002017]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT26532 WP_005053053.1 NC_017328:c398147-397836 [Shigella flexneri 2002017]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT26532 NC_017328:c398147-397836 [Shigella flexneri 2002017]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0