Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 298540..299234 Replicon chromosome
Accession NC_017328
Organism Shigella flexneri 2002017

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag SFXV_RS01510 Protein ID WP_001263491.1
Coordinates 298836..299234 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag SFXV_RS01505 Protein ID WP_000554759.1
Coordinates 298540..298833 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFXV_RS01485 (294179) 294179..294676 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
SFXV_RS01490 (294893) 294893..296605 - 1713 Protein_279 flagellar biosynthesis protein FlhA -
SFXV_RS01495 (296577) 296577..297362 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
SFXV_RS01500 (297433) 297433..298488 + 1056 WP_001226172.1 DNA polymerase IV -
SFXV_RS01505 (298540) 298540..298833 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
SFXV_RS01510 (298836) 298836..299234 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
SFXV_RS01515 (299244) 299244..299696 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
SFXV_RS01520 (299942) 299942..300985 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
SFXV_RS01525 (301047) 301047..301661 + 615 Protein_286 peptide chain release factor H -
SFXV_RS01530 (301718) 301718..303175 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
SFXV_RS01535 (303437) 303437..303895 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T26531 WP_001263491.1 NC_017328:298836-299234 [Shigella flexneri 2002017]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T26531 NC_017328:298836-299234 [Shigella flexneri 2002017]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT26531 WP_000554759.1 NC_017328:298540-298833 [Shigella flexneri 2002017]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT26531 NC_017328:298540-298833 [Shigella flexneri 2002017]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References