Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3510768..3511438 Replicon chromosome
Accession NZ_CP112997
Organism Mycobacterium tuberculosis variant bovis strain 2016/0386

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag ORO20_RS16595 Protein ID WP_003899954.1
Coordinates 3510768..3511112 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag ORO20_RS16600 Protein ID WP_003899955.1
Coordinates 3511109..3511438 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORO20_RS16560 (ORO20_16560) 3505841..3506701 + 861 WP_003416628.1 alpha/beta hydrolase -
ORO20_RS16565 (ORO20_16565) 3506676..3507191 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
ORO20_RS16570 (ORO20_16570) 3507207..3507418 + 212 Protein_3271 (R)-hydratase -
ORO20_RS16575 (ORO20_16575) 3507431..3507724 + 294 WP_003416635.1 hypothetical protein -
ORO20_RS16580 (ORO20_16580) 3508012..3509301 + 1290 WP_003416640.1 ATP-binding protein -
ORO20_RS16585 (ORO20_16585) 3509648..3510082 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
ORO20_RS16590 (ORO20_16590) 3510085..3510537 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
ORO20_RS16595 (ORO20_16595) 3510768..3511112 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ORO20_RS16600 (ORO20_16600) 3511109..3511438 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
ORO20_RS16605 (ORO20_16605) 3511976..3512323 + 348 WP_003899956.1 DUF2384 domain-containing protein -
ORO20_RS16610 (ORO20_16610) 3512320..3512940 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
ORO20_RS16615 (ORO20_16615) 3513100..3514365 - 1266 WP_003909837.1 hypothetical protein -
ORO20_RS16620 (ORO20_16620) 3514533..3514742 + 210 WP_003416778.1 hypothetical protein -
ORO20_RS16625 (ORO20_16625) 3514989..3516023 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T265093 WP_003899954.1 NZ_CP112997:3510768-3511112 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT265093 WP_003899955.1 NZ_CP112997:3511109-3511438 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References