Detailed information of TA system TA026500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 713092..713710
• Replicon: chromosome
• Accession: NC_017322
• Organism: Sinorhizobium meliloti BL225C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SINMEB_RS03365
• Protein ID: WP_010969011.1
• Coordinates: 713414..713710 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SINMEB_RS03360
• Protein ID: WP_010969010.1
• Coordinates: 713092..713400 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINMEB_RS03315	708171..708491	-	321	WP_003527320.1	YnfA family protein	-
SINMEB_RS33095	708566..708739	-	174	WP_010969006.1	hypothetical protein	-
SINMEB_RS33100	708753..708926	-	174	WP_003527318.1	hypothetical protein	-
SINMEB_RS03330	709162..709863	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
SINMEB_RS03335	710087..710428	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
SINMEB_RS03340	711013..711279	+	267	WP_003527312.1	sel1 repeat family protein	-
SINMEB_RS03345	711301..712422	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SINMEB_RS03355	712851..713081	-	231	WP_026029805.1	hypothetical protein	-
SINMEB_RS03360	713092..713400	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SINMEB_RS03365	713414..713710	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINMEB_RS03370	713758..714063	-	306	WP_003527305.1	hypothetical protein	-
SINMEB_RS03375	714391..715428	+	1038	WP_003527304.1	porin	-
SINMEB_RS03380	715538..716605	-	1068	WP_014526650.1	site-specific integrase	-
SINMEB_RS03385	716646..717695	-	1050	WP_010969013.1	porin	-
SINMEB_RS03390	717909..718397	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T26500 WP_010969011.1 NC_017322:c713710-713414 [Sinorhizobium meliloti BL225C]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T26500 NC_017322:c713710-713414 [Sinorhizobium meliloti BL225C]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT26500 WP_010969010.1 NC_017322:c713400-713092 [Sinorhizobium meliloti BL225C]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT26500 NC_017322:c713400-713092 [Sinorhizobium meliloti BL225C]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61