Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3532459..3533000 Replicon chromosome
Accession NZ_CP111008
Organism Escherichia coli strain J53-p130

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ORI87_RS17425 Protein ID WP_000615983.1
Coordinates 3532722..3533000 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ORI87_RS17420 Protein ID WP_000729703.1
Coordinates 3532459..3532719 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI87_RS17400 (3528134) 3528134..3528919 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ORI87_RS17405 (3528891) 3528891..3530603 + 1713 Protein_3406 flagellar biosynthesis protein FlhA -
ORI87_RS17410 (3530827) 3530827..3531324 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ORI87_RS17415 (3531500) 3531500..3532249 - 750 WP_000056849.1 C40 family peptidase -
ORI87_RS17420 (3532459) 3532459..3532719 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ORI87_RS17425 (3532722) 3532722..3533000 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ORI87_RS17430 (3533156) 3533156..3533896 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
ORI87_RS17435 (3533867) 3533867..3534634 - 768 WP_000333380.1 class II glutamine amidotransferase -
ORI87_RS17440 (3534840) 3534840..3535418 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T264789 WP_000615983.1 NZ_CP111008:3532722-3533000 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT264789 WP_000729703.1 NZ_CP111008:3532459-3532719 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References