Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3526262..3526956 Replicon chromosome
Accession NZ_CP111008
Organism Escherichia coli strain J53-p130

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ORI87_RS17385 Protein ID WP_001263489.1
Coordinates 3526262..3526660 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ORI87_RS17390 Protein ID WP_000554758.1
Coordinates 3526663..3526956 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3521850) 3521850..3521930 - 81 NuclAT_12 - -
- (3521850) 3521850..3521930 - 81 NuclAT_12 - -
- (3521850) 3521850..3521930 - 81 NuclAT_12 - -
- (3521850) 3521850..3521930 - 81 NuclAT_12 - -
ORI87_RS17360 (3522526) 3522526..3522984 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ORI87_RS17365 (3523245) 3523245..3524702 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ORI87_RS17370 (3524759) 3524759..3525280 - 522 Protein_3399 peptide chain release factor H -
ORI87_RS17375 (3525276) 3525276..3525482 - 207 Protein_3400 RtcB family protein -
ORI87_RS17380 (3525800) 3525800..3526252 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
ORI87_RS17385 (3526262) 3526262..3526660 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ORI87_RS17390 (3526663) 3526663..3526956 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ORI87_RS17395 (3527008) 3527008..3528063 - 1056 WP_001226164.1 DNA polymerase IV -
ORI87_RS17400 (3528134) 3528134..3528919 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ORI87_RS17405 (3528891) 3528891..3530603 + 1713 Protein_3406 flagellar biosynthesis protein FlhA -
ORI87_RS17410 (3530827) 3530827..3531324 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T264788 WP_001263489.1 NZ_CP111008:c3526660-3526262 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT264788 WP_000554758.1 NZ_CP111008:c3526956-3526663 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References