Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3532866..3533407 Replicon chromosome
Accession NZ_CP111004
Organism Escherichia coli strain J53-p131

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ORI86_RS17430 Protein ID WP_000615983.1
Coordinates 3533129..3533407 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ORI86_RS17425 Protein ID WP_000729703.1
Coordinates 3532866..3533126 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI86_RS17405 (3528541) 3528541..3529326 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ORI86_RS17410 (3529298) 3529298..3531010 + 1713 Protein_3406 flagellar biosynthesis protein FlhA -
ORI86_RS17415 (3531234) 3531234..3531731 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ORI86_RS17420 (3531907) 3531907..3532656 - 750 WP_000056849.1 C40 family peptidase -
ORI86_RS17425 (3532866) 3532866..3533126 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ORI86_RS17430 (3533129) 3533129..3533407 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ORI86_RS17435 (3533563) 3533563..3534303 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
ORI86_RS17440 (3534274) 3534274..3535041 - 768 WP_000333380.1 class II glutamine amidotransferase -
ORI86_RS17445 (3535247) 3535247..3535825 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3511955..3557754 45799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T264761 WP_000615983.1 NZ_CP111004:3533129-3533407 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT264761 WP_000729703.1 NZ_CP111004:3532866-3533126 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References