Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3526669..3527363 Replicon chromosome
Accession NZ_CP111004
Organism Escherichia coli strain J53-p131

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ORI86_RS17390 Protein ID WP_001263489.1
Coordinates 3526669..3527067 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ORI86_RS17395 Protein ID WP_000554758.1
Coordinates 3527070..3527363 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3522257) 3522257..3522337 - 81 NuclAT_12 - -
- (3522257) 3522257..3522337 - 81 NuclAT_12 - -
- (3522257) 3522257..3522337 - 81 NuclAT_12 - -
- (3522257) 3522257..3522337 - 81 NuclAT_12 - -
ORI86_RS17365 (3522933) 3522933..3523391 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ORI86_RS17370 (3523652) 3523652..3525109 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ORI86_RS17375 (3525166) 3525166..3525687 - 522 Protein_3399 peptide chain release factor H -
ORI86_RS17380 (3525683) 3525683..3525889 - 207 Protein_3400 RtcB family protein -
ORI86_RS17385 (3526207) 3526207..3526659 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
ORI86_RS17390 (3526669) 3526669..3527067 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ORI86_RS17395 (3527070) 3527070..3527363 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ORI86_RS17400 (3527415) 3527415..3528470 - 1056 WP_001226164.1 DNA polymerase IV -
ORI86_RS17405 (3528541) 3528541..3529326 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ORI86_RS17410 (3529298) 3529298..3531010 + 1713 Protein_3406 flagellar biosynthesis protein FlhA -
ORI86_RS17415 (3531234) 3531234..3531731 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3511955..3557754 45799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T264760 WP_001263489.1 NZ_CP111004:c3527067-3526669 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT264760 WP_000554758.1 NZ_CP111004:c3527363-3527070 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References