Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1983228..1983955 Replicon chromosome
Accession NZ_CP110996
Organism Escherichia coli strain XYEH3783

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ORI30_RS09510 Protein ID WP_000550189.1
Coordinates 1983641..1983955 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ORI30_RS09505 Protein ID WP_000560266.1
Coordinates 1983228..1983644 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ORI30_RS09495 (1978388) 1978388..1980739 + 2352 WP_000695487.1 alpha-glucosidase -
ORI30_RS09500 (1981165) 1981165..1983183 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ORI30_RS09505 (1983228) 1983228..1983644 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ORI30_RS09510 (1983641) 1983641..1983955 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ORI30_RS09515 (1984239) 1984239..1985375 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ORI30_RS09520 (1985460) 1985460..1985963 + 504 WP_001333820.1 M48 family metallopeptidase -
ORI30_RS09525 (1986040) 1986040..1986732 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
ORI30_RS09530 (1986811) 1986811..1987797 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T264694 WP_000550189.1 NZ_CP110996:c1983955-1983641 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT264694 WP_000560266.1 NZ_CP110996:c1983644-1983228 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References