Detailed information of TA system TA264683

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54376..54802
• Replicon: plasmid pYUXYEH3314-NDM
• Accession: NZ_CP110995
• Organism: Escherichia coli strain XYEH3314

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: ORI32_RS25305
• Protein ID: WP_001323520.1
• Coordinates: 54686..54802 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54376..54600 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ORI32_RS25270 (50342)	50342..50839	+	498	WP_095719660.1	single-stranded DNA-binding protein	-
ORI32_RS25275 (50902)	50902..51135	+	234	WP_000005990.1	DUF905 family protein	-
ORI32_RS25280 (51201)	51201..53159	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
ORI32_RS25285 (53214)	53214..53648	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ORI32_RS25290 (53645)	53645..54407	+	763	Protein_68	plasmid SOS inhibition protein A	-
ORI32_RS25295 (54376)	54376..54564	-	189	WP_001299721.1	hypothetical protein	-
- (54376)	54376..54600	+	225	NuclAT_0	-	Antitoxin
- (54376)	54376..54600	+	225	NuclAT_0	-	Antitoxin
- (54376)	54376..54600	+	225	NuclAT_0	-	Antitoxin
- (54376)	54376..54600	+	225	NuclAT_0	-	Antitoxin
ORI32_RS25300 (54586)	54586..54735	+	150	Protein_70	plasmid maintenance protein Mok	-
ORI32_RS25305 (54686)	54686..54802	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
ORI32_RS25310 (55022)	55022..55252	+	231	WP_001426396.1	hypothetical protein	-
ORI32_RS25315 (55250)	55250..55423	-	174	Protein_73	hypothetical protein	-
ORI32_RS25320 (55493)	55493..55699	+	207	WP_000547968.1	hypothetical protein	-
ORI32_RS25325 (55724)	55724..56011	+	288	WP_000107544.1	hypothetical protein	-
ORI32_RS25330 (56129)	56129..56950	+	822	WP_001234469.1	DUF932 domain-containing protein	-
ORI32_RS25335 (57247)	57247..57894	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
ORI32_RS25340 (58171)	58171..58554	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
ORI32_RS25345 (58745)	58745..59431	+	687	WP_000332484.1	PAS domain-containing protein	-
ORI32_RS25350 (59525)	59525..59752	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T264683 WP_001323520.1 NZ_CP110995:54686-54802 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT264683 NZ_CP110995:54376-54600 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin