Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3776403..3777097 Replicon chromosome
Accession NZ_CP110988
Organism Escherichia coli strain XYEH3314

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag ORI32_RS18395 Protein ID WP_001263493.1
Coordinates 3776403..3776801 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag ORI32_RS18400 Protein ID WP_000554757.1
Coordinates 3776804..3777097 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3772063) 3772063..3772143 - 81 NuclAT_11 - -
- (3772063) 3772063..3772143 - 81 NuclAT_11 - -
- (3772063) 3772063..3772143 - 81 NuclAT_11 - -
- (3772063) 3772063..3772143 - 81 NuclAT_11 - -
ORI32_RS18365 (3771403) 3771403..3772647 - 1245 WP_000189541.1 esterase FrsA -
ORI32_RS18370 (3772739) 3772739..3773197 - 459 WP_061349133.1 xanthine phosphoribosyltransferase -
ORI32_RS18375 (3773458) 3773458..3774915 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
ORI32_RS18380 (3774972) 3774972..3775493 - 522 Protein_3599 peptide chain release factor H -
ORI32_RS18385 (3775492) 3775492..3775695 - 204 Protein_3600 RtcB family protein -
ORI32_RS18390 (3775941) 3775941..3776393 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
ORI32_RS18395 (3776403) 3776403..3776801 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ORI32_RS18400 (3776804) 3776804..3777097 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ORI32_RS18405 (3777149) 3777149..3778204 - 1056 WP_001226164.1 DNA polymerase IV -
ORI32_RS18410 (3778275) 3778275..3779060 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
ORI32_RS18415 (3779032) 3779032..3780744 + 1713 Protein_3606 flagellar biosynthesis protein FlhA -
ORI32_RS18420 (3780968) 3780968..3781465 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T264678 WP_001263493.1 NZ_CP110988:c3776801-3776403 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT264678 WP_000554757.1 NZ_CP110988:c3777097-3776804 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References