264404

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3525794..3526488 Replicon chromosome
Accession NZ_CP110859
Organism Escherichia coli strain TSW4881-W1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag OQ464_RS17315 Protein ID WP_001263489.1
Coordinates 3525794..3526192 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OQ464_RS17320 Protein ID WP_000554758.1
Coordinates 3526195..3526488 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3521382) 3521382..3521462 - 81 NuclAT_12 - -
- (3521382) 3521382..3521462 - 81 NuclAT_12 - -
- (3521382) 3521382..3521462 - 81 NuclAT_12 - -
- (3521382) 3521382..3521462 - 81 NuclAT_12 - -
OQ464_RS17290 (3522058) 3522058..3522516 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
OQ464_RS17295 (3522777) 3522777..3524234 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
OQ464_RS17300 (3524291) 3524291..3524812 - 522 Protein_3380 peptide chain release factor H -
OQ464_RS17305 (3524808) 3524808..3525014 - 207 Protein_3381 RtcB family protein -
OQ464_RS17310 (3525332) 3525332..3525784 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
OQ464_RS17315 (3525794) 3525794..3526192 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OQ464_RS17320 (3526195) 3526195..3526488 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OQ464_RS17325 (3526540) 3526540..3527595 - 1056 WP_001226164.1 DNA polymerase IV -
OQ464_RS17330 (3527666) 3527666..3528451 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OQ464_RS17335 (3528423) 3528423..3530135 + 1713 Protein_3387 flagellar biosynthesis protein FlhA -
OQ464_RS17340 (3530359) 3530359..3530856 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T264404 WP_001263489.1 NZ_CP110859:c3526192-3525794 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT264404 WP_000554758.1 NZ_CP110859:c3526488-3526195 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References