Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3589845..3590539 Replicon chromosome
Accession NZ_CP110814
Organism Escherichia coli strain THB42-F4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N0552_RS17575 Protein ID WP_001263489.1
Coordinates 3589845..3590243 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N0552_RS17580 Protein ID WP_000554758.1
Coordinates 3590246..3590539 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3585433) 3585433..3585513 - 81 NuclAT_12 - -
- (3585433) 3585433..3585513 - 81 NuclAT_12 - -
- (3585433) 3585433..3585513 - 81 NuclAT_12 - -
- (3585433) 3585433..3585513 - 81 NuclAT_12 - -
N0552_RS17550 (3586109) 3586109..3586567 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N0552_RS17555 (3586828) 3586828..3588285 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N0552_RS17560 (3588342) 3588342..3588863 - 522 Protein_3431 peptide chain release factor H -
N0552_RS17565 (3588859) 3588859..3589065 - 207 Protein_3432 RtcB family protein -
N0552_RS17570 (3589383) 3589383..3589835 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N0552_RS17575 (3589845) 3589845..3590243 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N0552_RS17580 (3590246) 3590246..3590539 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N0552_RS17585 (3590591) 3590591..3591646 - 1056 WP_001226164.1 DNA polymerase IV -
N0552_RS17590 (3591717) 3591717..3592502 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0552_RS17595 (3592474) 3592474..3594186 + 1713 Protein_3438 flagellar biosynthesis protein FlhA -
N0552_RS17600 (3594410) 3594410..3594907 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T264311 WP_001263489.1 NZ_CP110814:c3590243-3589845 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT264311 WP_000554758.1 NZ_CP110814:c3590539-3590246 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References